Genetic transformation of the medicinal plant Salvia runcinata L. f. using Agrobacterium rhizogenes
نویسندگان
چکیده
منابع مشابه
Agrobacterium rhizogenes-Mediated Transformation of the Parasitic Plant Phtheirospermum japonicum
BACKGROUND Plants within the Orobanchaceae are an agriculturally important group of parasites that attack economically important crops to obtain water and nutrients from their hosts. Despite their agricultural importance, molecular mechanisms of the parasitism are poorly understood. METHODOLOGY/PRINCIPAL FINDINGS We developed transient and stable transformation systems for Phtheirospermum jap...
متن کاملGenetic transformation of the medicinal plant Ruta graveolens L. by an Agrobacterium tumefaciens-mediated method
Ruta graveolens L. is a source of pharmacologically active molecules such as furocoumarins, furoquinolines and acridone alkaloids. A genetic transformation method using Agrobacterium tumefaciens was developed for this plant species. The conditions for an efficient regeneration of R. graveolens led us to retain hypocotyls among other explants tested. Subsequently, transformation was obtained by ...
متن کاملAgrobacterium rhizogenes - Mediated Transformation of Medicinal Plants from the Family Rhamnaceae
A number of plants can be successfully transformed by Agrobacterium rhizogenes through the transfer of T-DNA from agrobacteria to the plant genome. Transgenic tissue – hairy roots – is produced as a result of the transformation process. This organized, genetically stable, hormone-independent transformed tissue is capable of accomplishing complex metabolic pathways, including biosynthesis and ac...
متن کاملThe Agrobacterium rhizogenes GALLS gene encodes two secreted proteins required for genetic transformation of plants.
Agrobacterium tumefaciens and Agrobacterium rhizogenes are related pathogens that cause crown gall and hairy root diseases, which result from integration and expression of bacterial genes in the plant genome. Single-stranded DNA (T strands) and virulence proteins are translocated into plant cells by a type IV secretion system. VirD2 nicks a specific DNA sequence, attaches to the 5' end, and pil...
متن کاملAn efficient protocol for peanut (Arachis hypogaea L.) transformation mediated by Agrobacterium rhizogenes
The Agrobacterium-mediated transformation of hairy roots creates a rapid and simple means to introduce and express foreign genes in plant cells that are capable of synthesizing specific natural products. We describe a method for the production of transgenic hairy root cultures of peanut (Arachis hypogaea L.) using Agrobacterium rhizogenes R1000, a strain with the binary vector pBI121. We evalua...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: South African Journal of Botany
سال: 2017
ISSN: 0254-6299
DOI: 10.1016/j.sajb.2017.05.029